The Bundesanstalt für Materialforschung und -prüfung (BAM), in cooperation with the Netherlands Organization for Applied Scientific Research (TNO), is working on a framework for integrating frequently updated structural models into an asset management process for bridge structures. A multi-span steel road bridge was selected as a test case for the development of this framework. In order for the structural model to represent the real behavior of the bridge with sufficient accuracy, model calibration is required. In this case, we have planned to calibrate the model based on the dynamic response of the bridge. To determine its dynamic properties, a multi-setup operational modal analysis was performed on one of the bridge spans. In parallel, a structural model of the span was developed based on the available design and service life information. Both eigenfrequencies and mode shapes were used as reference parameters to calibrate the model. A sensitivity analysis was performed to identify the most influential design parameters. Subsequently, a genetic algorithm was applied for minimizing the difference between measured and simulated characteristic responses. In the proposed paper, we summarize the measurements as well as the determination of the modal response of the bridge and describe the process of calibration of the structural model using the identified dynamic response.